Review Questions

1. What is the specific role of the secretory pathway in nerve cells?

 

2. What are vesicles and what are their two primary components?

 

3. Define the term “membrane fusion” with respect to vesicular transport.

 

4. Which of the membranes discussed in the lecture are characteristic of the secretory pathway in yeast? Are these membranes found only in yeast cells?

 

5. What role does the secretory pathway play in actively growing (budding) yeast?

 

6. Why is the Golgi apparatus considered the “bus station” of the cell?

 

7. What does it mean to say that a mutation is “temperature sensitive?” Why are such mutations helpful for studying essential genes?

 

8. Describe the phenotype of sec1 mutants grown at 37°C (body temperature). What does this imply about the role of Sec1p in the secretory pathway?

 

9. Describe another secretory mutant phenotype distinct from the sec1 mutant phenotype. What does this imply about the role of the mutated genes in the secretory pathway?