Review Questions

1. Review how protein kinases work, and explain how the analog-sensitive kinase mutant system allows identification of kinase substrates in a crude cell extract containing many different kinases.


2. Explain how the analog-sensitive kinase mutant system allows the construction of a cell line in which a single protein kinase can be specifically inhibited by addition of a chemical to the medium.


3. Describe some of the cellular processes that Cdks regulate as the cell proceeds through the various stages of the cell cycle.


4. Explain how cyclins might influence the physiological function of the associated Cdk.


5. Discuss some of the mechanisms by which phosphorylation changes protein function.

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